Presentation is loading. Please wait.

Presentation is loading. Please wait.

Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice  Abbey Schepers, MD, Nuno M.M. Pires,

Published byΦαραώ Παπανδρέου Modified over 5 years ago

Similar presentations

Presentation on theme: "Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice  Abbey Schepers, MD, Nuno M.M. Pires,"— Presentation transcript:

1 Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice  Abbey Schepers, MD, Nuno M.M. Pires, MSc, Daniel Eefting, MD, Margreet R. de Vries, BSc, J. Hajo van Bockel, MD, PhD, Paul H.A. Quax, PhD  Journal of Vascular Surgery  Volume 43, Issue 4, Pages (April 2006) DOI: /j.jvs Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Dexamethasone treatment (0.15 mg · kg−1 · d−1 in drinking water) reduces vein graft thickening in hypercholesterolemic ApoE3Leiden mice after 28 days of treatment. A, Quantification of vein graft thickening shows a reduction of 43% in the dexamethasone-treated animals as compared with controls (n = 6; ⁎P < .05), whereas no significant changes in luminal area were observed (B). C, Representative cross section; arrows indicate a thickened vessel wall (stain, hematoxylin-phloxin-saffron; original magnification, ×40). No differences were seen in the cellular composition of thickened grafts in either group, as determined by computer-assisted morphometric analysis. D, Alpha smooth muscle actin staining representing vascular smooth muscle cells. E, AIA31240 staining representing macrophages. n.s., Not significant; dexa, dexamethasone. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Effect of 7 days of dexamethasone treatment on vein graft thickening 28 days after surgery in hypercholesterolemic ApoE3Leiden mice. There was a significant inhibition of vein graft thickening in the 7-day-treated animals (A) when compared with controls (n = 6; ⁎P < .05). There was no significant difference in luminal area between groups (B). n.s., Not significant; dexa, dexamethasone. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Effect of dexamethasone treatment on the early messenger RNA (mRNA) expression of proinflammatory cytokines in injured vessels of ApoE3Leiden mice (n = 4 per group). The relative mRNA expression in time in the dexamethasone-treated group is depicted compared with mRNA expression the control group (expression in the control group was defined as 1; ⁎P < .05). MCP, Monocyte chemotactic protein; TNF, tumor necrosis factor; MIP, macrophage inflammatory protein; TLR, toll-like receptor. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Effect of either short-term (D7d) or prolonged (D28d) dexamethasone exposure (0.75 mg/mL of medium) in intimal hyperplasia formation in human saphenous vein organ cultures (n = 12 per group). In both dexamethasone-exposed groups, a significantly decreased amount of intimal hyperplasia was seen when compared with the control segments (⁎P < .05; original magnification, ×100; dotted line indicates the border separating media from intima). n.s., Not significant. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Download ppt "Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice  Abbey Schepers, MD, Nuno M.M. Pires,"

Similar presentations

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,
Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.

Vein wall re-endothelialization after deep vein thrombosis is improved with low- molecular-weight heparin  Daria K. Moaveni, MD, Erin M. Lynch, MS, Cathy.
Inhibition of smooth muscle cell migration and neointima formation in vein grafts by overexpression of matrix metalloproteinase-3  Klaus Kallenbach, MD,

Inhibition of smooth muscle cell migration and neointima formation in vein grafts by overexpression of matrix metalloproteinase-3  Klaus Kallenbach, MD,
Decellularized vein as a potential scaffold for vascular tissue engineering  Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

Decellularized vein as a potential scaffold for vascular tissue engineering  Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Kathleen D. Gibson, MD, Brian L

Kathleen D. Gibson, MD, Brian L
Pharmacologic blockade and genetic deletion of androgen receptor attenuates aortic aneurysm formation  John P. Davis, MD, Morgan Salmon, PhD, Nicolas.

Pharmacologic blockade and genetic deletion of androgen receptor attenuates aortic aneurysm formation  John P. Davis, MD, Morgan Salmon, PhD, Nicolas.
Kenneth M. DeSart, MD, Khayree Butler, MD, Kerri A

Kenneth M. DeSart, MD, Khayree Butler, MD, Kerri A
Antisense oligonucleotide to proto-oncogene c-myb inhibits the formation of intimal hyperplasia in experimental vein grafts  Gregory J. Fulton, MB, FRCSI,

Antisense oligonucleotide to proto-oncogene c-myb inhibits the formation of intimal hyperplasia in experimental vein grafts  Gregory J. Fulton, MB, FRCSI,
Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 

Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 
Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R

Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R
Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries  Jens W Fischer,
Lysyl oxidase expression in a rat model of arterial balloon injury

Lysyl oxidase expression in a rat model of arterial balloon injury
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling 

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling 
Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,
True extracranial carotid artery aneurysm in a child

True extracranial carotid artery aneurysm in a child

Similar presentations

Ads by Google