1. (A) qRT-PCR-analysis of P450 aromatase mRNA in brains from control and d-Asp-treated frogs.
(A) qRT-PCR-analysis of P450 aromatase mRNA in brains from control and d-Asp-treated frogs. The mRNA levels are expressed relative to the control group and are normalised to the ribosomal protein P1 mRNA levels. Values shown represent the means ± s.d. of five samples. (B) The upper panel shows western blot detection of P450 aro protein in brains from control and d-Asp-treated frogs. A specific band was observed at a size of 58 kDa (by comparison with co-migrating size markers; Bio-Rad, Melville, NY, USA). The lower panel shows the amount of P450 aro protein quantified using the Image J program and normalised with respect to β-actin (see upper panel). Values shown represent the means ± s.d. of five samples (two bands shown in upper panel). *P<0.05 vs controls.
Lavinia Burrone et al. J Exp Biol 2012;215:
© 2012.