1. Optimization of a MYC degradation screen.
Optimization of a MYC degradation screen. (A) Schematic of the GPS-MYC screen. (B) GPS-MYC cells were treated with vehicle alone (DMSO) or with MG132 or CHX for 6 hours and analyzed on an IntelliCyt iQue Screener. The data presented are from the first two and last two wells of each control, representing the beginning (0 min) and the end (45 min) of the assay. Data are from one plate and are representative of at least three independent experiments. (C) The GPS-MYC screen was run in duplicate. Data were normalized to control DMSO (blue circles, 0% stabilization) and MG132 (green circles, 100% stabilization), and hits were determined by a cutoff of a 30% average stabilization of the two replicates. The circles representing the hits are shown in purple. The circle size is proportional to the number of events per well. Stabilizing compounds that were evaluated in the paper are labeled in the graph. (D) As described in (C), except the data were normalized to control DMSO (blue circles, 0% destabilization) and CHX (red circles, 100% destabilization). Destabilizing compounds evaluated in the paper are indicated.
Devon R. Blake et al., Sci. Signal. 2019;12:eaav7259
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