1. Fibril-induced NM prions do not evolve from SGs.
Fibril-induced NM prions do not evolve from SGs. N2a NM-HAsol cells were incubated with 5 μM recombinant NM fibrils (monomer concentration) for the indicated time points and subsequently analyzed for NM-HA aggregate induction and SGs. As a positive control, cells were incubated with 0.5 mM sodium arsenite for 1 h without addition of fibrils. Immunofluorescence staining was performed using mAb anti-HA (green) and pAb anti-TIAR (red). Nuclei were stained with Hoechst (blue). Scale bar: 10 μm.
Katrin Riemschoss et al. LSA 2019;2:e
© 2019 Riemschoss et al.