1. Comparison of rDNA histone modifications between mESCs and mNPCs.
Comparison of rDNA histone modifications between mESCs and mNPCs. (A) ChIP-seq plots comparing the distributions of H3K4me1, H3K4me2, H3K4me3, H3K9me3, H3K27me3, and H3K36me3 at rDNA in mESCs, mNPCs, and MEFs. Signal intensity as determined by F-seq is plotted on the Y-axis. (B) Normalized tag density scores for histone modifications at rDNA in mNPCs and MEFs. (C) ChIP-PCR evaluation of H3K4me1 enrichment at rDNA in mESCs and mNPCs. Enrichment values were normalized to the +6859/+6980 negative control amplicon. Error bars represent mean ± SD for triplicates. (D) Pre-rRNA levels in mESCs and mNPCs as measured by qRT-PCR. Mean ± SEM is shown (n = 2). *P = by one-tailed t test.
Gabriel E. Zentner et al. G3 2013;4:
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