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Figure 1 EAE severity and CNS pathology in Dex-treated MIF−/− and Wt mice Wild-type (Wt) and macrophage migration inhibitory factor (MIF)−/− mice were.

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Presentation on theme: "Figure 1 EAE severity and CNS pathology in Dex-treated MIF−/− and Wt mice Wild-type (Wt) and macrophage migration inhibitory factor (MIF)−/− mice were."— Presentation transcript:

1 Figure 1 EAE severity and CNS pathology in Dex-treated MIF−/− and Wt mice Wild-type (Wt) and macrophage migration inhibitory factor (MIF)−/− mice were immunized to induce experimental autoimmune encephalomyelitis (EAE) as described in the Methods. EAE severity and CNS pathology in Dex-treated MIF−/− and Wt mice Wild-type (Wt) and macrophage migration inhibitory factor (MIF)−/− mice were immunized to induce experimental autoimmune encephalomyelitis (EAE) as described in the Methods. Mice were injected intraperitoneally with (A) 5 mg/kg dexamethasone (Dex) vs phosphate-buffered saline (PBS) control every day starting on day 6 and then every other day after onset until the peak of disease (Wt PBS, n = 12; Wt Dex, n = 11; MIF−/− PBS, n = 12; MIF−/− Dex, n = 10) or (B) 10 mg/kg Dex vs PBS control for 3 continuous days upon onset of disease (Wt PBS, n = 12; Wt Dex, n = 13; MIF−/− PBS, n = 12; MIF−/− Dex, n = 12). Mice were scored daily for EAE as described in the Methods. Shown are pooled data from 2 to 3 independent experiments (mean ± SEM). *indicates significant difference between MIF−/− Dex group and Wt PBS group or MIF−/− PBS group; †indicates significant difference between MIF−/− Dex group and Wt Dex group (analysis of variance [ANOVA], p < 0.05). EAE mice from (B) were sacrificed for analysis (C) at the acute stage (day 16–24 postimmunization, whenever PBS groups reached clinical score of 2 or higher) of disease and then quantified for CD11b+, CD11c+, CD4+, and CD8+ cellular infiltration in the brain by flow cytometry. Shown are pooled data from 3 independent experiments (mean ± SEM; n = 7–8 mice per group; ANOVA, no significant difference; post hoc Mann-Whitney test for indicated groups; *p < 0.05). NS = not significant. Niannian Ji et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e139 © 2015 American Academy of Neurology

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