1. Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources.
Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources. (A) Expression of the six GAP genes was analyzed in the BSC1 (wild type) and the csy1 (CAEB-5) mutant strain. Nitrogen-starved cells were resuspended in YNB medium supplemented with 5 mg/ml of ammonium sulfate supplemented with 100 µg/ml histidine, and samples were taken after 15 (gray bars) or 30 min (white bars). The fold change in gene expression of the GAP genes in the csy1 mutant relative to expression in the wild-type strain is shown. (B) The fold changes in gene expression levels between GAP genes when cells were shifted from nitrogen starvation medium to YPD medium. (C) Fold changes in gene expression levels between GAP genes when cells were shifted from YPD medium to nitrogen starvation medium. Gray bars show expression at 15 min and white bars show levels at 30 min, on the basis of shifting to the new medium (for panels B and C). The comparative CT method to calculate relative changes in gene expression was used, with TEF1 as the housekeeping gene. Error bars show the range of possible relative quantity values defined by the standard errors of the ΔΔCT values. All experiments were evaluated for at least three RNA preparations.
Lucie Kraidlova et al. mSphere 2016; doi: /mSphere