1. Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P
Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P. pastoris A2AR–SMALP (●) and DDM-solubilized A2AR (▲) prepared from P. pastoris overexpressing A2aR were incubated for 30 min at the stated temperatures, chilled on ice, before specific binding o...
Thermostability of A2AR–SMALP and DDM-solubilized A2AR from P. pastoris A2AR–SMALP (●) and DDM-solubilized A2AR (▲) prepared from P. pastoris overexpressing A2aR were incubated for 30 min at the stated temperatures, chilled on ice, before specific binding of [3H]ZM was determined as described in ‘Methods’. Data are expressed as specific binding relative to the 20°C data point (mean ± S.E.M. of three separate experiments performed in triplicate).
Mohammed Jamshad et al. Biosci. Rep. 2015;35:e00188
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