1. Cell cycle release after M phase arrest (h)
Supplementary Fig. S6 a b
ZNF313
p21WAF1
Cyclin D1
Cyclin B1
CDK6
Tubulin
Serum addition (1 h)
Serum withdrawal
Thymidine block
Nocodazole
Control
G1 S G2/M arrest
Control
Thymidine
block
Serum addition (1 h)
Nocodazole
Serum
withdrawal c d
Control
HeLa : M pahse arrest by thymidine-nocodazole-mitotic shake-off method.
Cell cycle release after M phase arrest (h)
C (h)
ZNF313
p21WAF1
Cyclin B1
Tubulin
Hours after 10% FBS addition (HCT116) G1 S
G2/M e f
ZNF313
p21WAF1
Tubulin
Control
10% FBS
IGF-1
EGF
FGF
A549
pcDNA WT-ZNF313
HeLa HCT116
70.1
13.2
16.7
48.2
31.3
20.5
50.3
21.6
18.1
41.1
33.3
25.6
G1 (%) S
G2/M
52.8
15.8
31.4
67.5
10.3
22.2
PC HCT116
siControl siZNF313-1
73.9
11.3
14.8
55.4
15.2
29.4