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FUT1 and STAM are required for the retrograde transport of STx2B to the Golgi. FUT1 and STAM are required for the retrograde transport of STx2B to the.

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Presentation on theme: "FUT1 and STAM are required for the retrograde transport of STx2B to the Golgi. FUT1 and STAM are required for the retrograde transport of STx2B to the."— Presentation transcript:

1 FUT1 and STAM are required for the retrograde transport of STx2B to the Golgi.
FUT1 and STAM are required for the retrograde transport of STx2B to the Golgi. (A) RT–PCR. (B) Immunofluorescence to detect LC3. Scale bar, 25 μm. (C) LC3 punctae per cell from (B). N > 15 cells per condition. *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for the comparison between control transfection and other groups. (D) STx2B transport assays. Scale bars, 25 μm. (E) STx2B levels from (D). Levels in control transfected cells at 60 min were normalized to 100. N > 20 cells per condition. *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for the comparison between control transfection and other groups. (F, G) Immunofluorescence in WT or ΔUNC50 cells (F), or WT cells transfected with control or GBF1 siRNA (G). Scale bars, 25 μm. Andrey S Selyunin et al. LSA 2019;2:e © 2019 Selyunin et al.


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