1. Somatic mutational analysis of DAX1 in testes from men with idiopathic azoospermia 
Giovanna Mantovani, M.D., Mario Mancini, M.D., Giacomo Gazzano, M.D., Anna Spada, M.D., Giovanni M. Colpi, M.D., Paolo Beck- Peccoz, M.D., Luca Persani, M.D., Ph.D. 
Fertility and Sterility 
Volume 84, Issue 5, Pages (November 2005)
DOI: /j.fertnstert
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
(A) Reverse transcriptase polymerase chain reaction (RT-PCR) analysis of DAX-1 transcript in seven testicular samples from azoospermic patients. A full-length transcript was detected in all tissues. (B) Representative samples: immunostaining (magnification ×10) for DAX1 performed in one Sertoli cell only syndrome (SCOS) sample, one maturation arrest sample, and one spermatogenic hypoplasia sample. Tissue sections were incubated with a specific monoclonal antibody directed against the DAX1 C-terminus. The immunoreactivity was visualized by diaminobenzidine; positive cells had a brown color at the site of reaction. The arrow indicates a positively stained extratubular Leydig cell.
Mantovani. DAX1 somatic mutations in azoospermia. Fertil Steril 2005.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions