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Fig. 3. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. Inclusion of E-cadherin into stationary clusters.

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Presentation on theme: "Fig. 3. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. Inclusion of E-cadherin into stationary clusters."— Presentation transcript:

1 Fig. 3. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. (A) Schematic diagram and table of mutants. (B,C) FRAP analysis of wild-type Ecad-GFP and mutants expressed in PDAC (B) and L-cells (C). Deletion of any single interaction reduces Fi from the level of wild-type E-cadherin (∼60%) to the level of the non-interacting mutant ΔEC1ΔCyt (∼30%), indicating that all three interactions, cis-, trans-, and cytoplasmic, are required for inclusion of Ecad-GFP into stationary adhesive clusters. Those mutants retaining actin association (trans-, cis-, and ΔEC1 mutants) recover more slowly than mutants lacking the cytoplasmic domain (ΔEC1ΔCyt, and ΔCyt). Retention of cis- and trans- interactions by the ΔCyt mutant did not significantly slow its recovery compared to the ΔEC1ΔCyt mutant. Values for Ecad-GFP and ΔEC1ΔCyt are included from Fig. 2C for comparison; see supplementary material Table S2 for list of all FRAP parameters. (D) TEER measured in L-cells expressing low and high levels of wild-type E-cadherin, and E-cadherin mutants. Note that none of the mutants were able to increase the effective cell adhesion strength as assessed by electrical resistance above the level of L-cells, which did not express E-cadherin. N=3 for each condition; error bars represent s.e.m. Zahra Erami et al. Biology Open 2015;bio © Published by The Company of Biologists Ltd

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