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Fig. 7. Rho family GTPase signaling is required for cyst formation in Ptp4E Ptp10D mutants.(A–C) Expression of DN Rho1 and Rac1 mutants in wild-type (w1118) embryos produced defects in tracheal branching, including failures of GB extension (B,C, arrows). Rho family GTPase signaling is required for cyst formation in Ptp4E Ptp10D mutants.(A–C) Expression of DN Rho1 and Rac1 mutants in wild-type (w1118) embryos produced defects in tracheal branching, including failures of GB extension (B,C, arrows). In the Ptp4E Ptp10D background (D), expression of Rho1-DN (E) or Rac1-DN (not shown) produced complete suppression of the TC/LT diameter phenotype, and partial suppression of the GB cyst number phenotype. Cdc42-DN (F) and Rho1 dsRNA (not shown) produced partial suppression of the TC/LT phenotype, but did not affect GB cyst number. In all panels, arrowheads indicate the positions of TC/LT junctions. (G,H) Quantitative analysis of TC/LT (G) and GB cyst number (H) phenotypes. Scale bar: 10 µm. Mili Jeon et al. Biology Open 2012;bio © Published by The Company of Biologists Ltd
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