1. AKAP79 preferentially interacts with and is palmitoylated by DHHC2.
AKAP79 preferentially interacts with and is palmitoylated by DHHC2. A, Anti-GFP co-IP of the indicated myc-tagged DHHC-family proteins with AKAP79-GFP in HEK293 cells; myc-DHHC2 precipitates robustly with AKAP79-GFP. B, The N-terminal basic polybasic membrane targeting region of AKAP79 (1–153) is sufficient for co-IP with DHHC2 and the C36,129S mutation prevents this interaction. C, Both HA-DHHC2WT and catalytically inactive HA-DHHC2CS co-IP with AKAP79WT-GFP. D, AKAP79 palmitoylation is increased by DHHC2WT but not DHHC2CS in COS7 cells as revealed by the BMCC-biotin palmitoylation assay. E, Quantification reveals higher levels of palmitoylated AKAP79-GFP when mCh-tagged DHHC2WT but not catalytically inactive DHHC2CS is coexpressed normalized to control conditions expressing AKAP79-GFP and mCH alone. AKAP79CS serves as negative control for assay specificity in D and E (n = 5; data represented as mean ± SEM; *p < 0.05, one-way ANOVA, Dunnett's post hoc test).
Kevin M. Woolfrey et al. J. Neurosci. 2015;35:
©2015 by Society for Neuroscience