1. Genome Organization and Function: A View from Yeast and Arabidopsis
Sáez-Vásquez Julio , Gadal Olivier  
Molecular Plant 
Volume 3, Issue 4, Pages (July 2010)
DOI: /mp/ssq034
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Heterochromatic Chromocenters in 3-Week-Old A. thaliana Plants.
The condensate chromocenters structures, which contain mainly repetitive DNA sequences, can be visualized by DAPI staining (white signal) and the 45S and 5S rDNA genes (green and red signal, respectively) by FiSH. Counterstained with DAPI, FiSH with 5S and 45S and merged images are shown. Courtesy of Dr Tourmente and Douet (U. Blaise Pascal-Aubière, France).
Molecular Plant 2010 3, DOI: ( /mp/ssq034)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

3. Figure 2 Model of Regulation via Spatial Positioning.
Nucleus (wire-frame gray sphere) is a highly organized compartment, in which regulatory elements are concentrated in focus (black domains). From this focus, a concentration gradient is established generating a local environment, defined here in the Cartesian coordinate system, with the center of the focus as origin and axis lines X, Y, and Z. The same gene can be activated (green) or repressed (red), depending only on its spatial coordinate (from x,y,z to x’,y’,z’), changing its position relative to the regulatory domain.
Molecular Plant 2010 3, DOI: ( /mp/ssq034)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

4. Figure 3 Yeast Nuclear Organization.
(A) Ultrastructure of cryo-fixed, cryosubstituted yeast nucleus (strain NOY1071). Spindle pole body (SPB, black arrow), organize intranuclear microtubule (μtubules, white arrow) and is diametrically opposed to the nucleolus (No).
(B) Confocal section of a yeast nucleus with FROS insertion in GAL1 locus. GFP signal corresponds to nuclear pore complex (NPC) and gene labeling (green) mCherry-Nop1, the yeast orthologs of fibrillarin label the nucleolus (red). Scale bar = 200 nm.
(C) Schematic representation of yeast chromosome organization. Spindle pole body (SPB) anchors centromeres (CEN) via microtubules, diametrically opposed to the nucleolus (No). Chromosome (blue lines) are concentrated in the nucleoplasm (Np). Telomeres are forming cluster (TEL) at the nuclear periphery near the nuclear pore. Courtesy of Dr Isabelle Leger-Silvestre (EM-section, LBME-CNRS, Universite Paul-Sabatier).
Molecular Plant 2010 3, DOI: ( /mp/ssq034)
Copyright © 2010 The Authors. All rights reserved. Terms and Conditions