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Representative example of gating on T-cell subpopulations.

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Presentation on theme: "Representative example of gating on T-cell subpopulations."— Presentation transcript:

1 Representative example of gating on T-cell subpopulations.
Representative example of gating on T-cell subpopulations. (A) Flow cytometric plot of collected cells from a healthy control upon SEB stimulation with forward scatter and cells binding CD3 antibodies. Gate settings on CD3 cells are indicated. (B) Examination of subpopulations of gated T-cells (CD3) from a healthy control after whole blood stimulation with SEB, EBNA1, EBV-EA/D and phosphate-buffered saline. B(i) Dot plot of CD8 and CD69 T-cells. B(ii) Dot plot of CD8 and IFN-γ-producing T-cells. B(iii) Dot plot of CD69 and IFN-γ-producing T-cells. SEB, staphylococcal enterotoxin B; EBNA1, Epstein–Barr virus nuclear antigen 1; EBV-EA/D, Epstein–Barr virus early antigen diffuse; IFN, interferon. Anette Holck Draborg et al. Lupus Sci Med 2014;1:e000015 ©2014 by Lupus Foundation of America

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