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Nuclear localization of Sfl1.
Nuclear localization of Sfl1. Overnight-cultured cells of CAI4+ pBA1-GFP (GFP) or CAI4+ pBA1-SFL1-GFP (Sfl1-GFP) were diluted in fresh YPD at 25°C for yeast growth (A) and in YPD plus 10% serum at 37°C for hyphal growth (B). Cells were resuspended in phosphate-buffered saline and stained with 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for fluorescence observation. DIC, differential interference contrast. Yandong Li et al. Eukaryotic Cell 2007; doi: /EC
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