1. A LAT-dependent amino acid uptake system operates in both osteoclasts and osteoblasts.
A LAT-dependent amino acid uptake system operates in both osteoclasts and osteoblasts. (A) Schematic diagram of the procedure for generating [125I]IMT. (B) Primary osteoblasts and osteoclasts cultured from wild-type (WT) mice were incubated with [125I]IMT at 4° or 37°C for 30 min in HBSS buffer. n = 4 cell cultures from different mice. (C to F) Primary cells were incubated with [125I]IMT at 37°C for 30 min in HBSS buffer in which NaCl was replaced with equimolar ChoCl (C and D) or in HBSS buffer containing JPH203 (E and F). n = 4 cell cultures from different mice. Data were analyzed by the two-tailed Student’s t test. *P < 0.05 and **P < 0.01, significantly different from the value obtained in cells incubated at 4°C (B), cells incubated in HBSS buffer with NaCl (C and D), or cells incubated in HBSS buffer with dimethyl sulfoxide (DMSO) (E and F).
Kakeru Ozaki et al., Sci. Signal. 2019;12:eaaw3921
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