1. Effect of high glucose and agents that alter mitochondrial metabolism on ROS production and expression of COX-2 mRNA in HMCs. Cells were incubated with 5.6 mmol/l glucose, 30 mmol/l glucose, or 30 mmol/l glucose plus TTFA, CCCP, UCP-1, or MnSOD.
Effect of high glucose and agents that alter mitochondrial metabolism on ROS production and expression of COX-2 mRNA in HMCs. Cells were incubated with 5.6 mmol/l glucose, 30 mmol/l glucose, or 30 mmol/l glucose plus TTFA, CCCP, UCP-1, or MnSOD. A: ROS production was quantified using the fluorescent probe C-2938 (n = 9). B: Expression of COX-2 mRNA was quantified by a real-time RT-PCR method (n = 4). Results are expressed as values relative to 5.6 mmol/l glucose as 100% (mean ± SE). *P < 0.05 vs. cells incubated with 5.6 mmol/l glucose; #P < 0.05 vs. cells incubated with 30 mmol/l glucose.
Shinsuke Kiritoshi et al. Diabetes 2003;52:
©2003 by American Diabetes Association