1. PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded skin.
PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded skin. A–C: Day 7 wound tissue. Representative skin sections of Wnt-reporter BAT-gal mice were stained with X-gal after the injection of Ad-PEDF or Ad-GFP: resting skin stained with X-gal (A); wounded skin stained with X-gal after the Ad-GFP injection (B); and wound skin stained with X-gal after the Ad-PEDF injection (C). Scale bar = 200 μm. D: Western blot analysis of p-LRP6 and non-p-β-catenin in wounded skin after injecting Ad-PEDF or Ad-GFP into the wound bed at day 4. G: Western blot analysis of p-LRP6 and non-p-β-catenin in wound tissue 7 days after injection of Ad-GFP and Ad-PEDF into the wound bed. J: Western blot analysis of p-LRP6 and non-p-β-catenin in wound tissue of WT vs. PEDF KO mice at day 7 after being wounded. E, F, H, I, K, and L: The Western blotting results were semiquantified by densitometry, normalized by β-actin levels, averaged in three independent experiments, and expressed as a percentage of the respective control. All values are the mean ± SD (n = 9, **P < 0.01). Statistical significance was considered at P < All data were expressed as the mean ± SD.
Weiwei Qi et al. Diabetes 2015;64:
©2015 by American Diabetes Association