1. Differential expression of microRNAs in exhaled breath condensates of patients with asthma, patients with chronic obstructive pulmonary disease, and healthy adults 
Mark Pinkerton, BS, Vernon Chinchilli, PhD, Erin Banta, MD, Timothy Craig, DO, Avery August, PhD, Rebecca Bascom, MD, Margherita Cantorna, PhD, Eric Harvill, PhD, Faoud T. Ishmael, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 132, Issue 1, Pages e2 (July 2013)
DOI: /j.jaci
Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
Box plots showing the expression of specific miRNAs (as RNA copy number/mL of EBC) in patients with asthma, COPD, or healthy controls. *P < .05.
Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci )
Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3. Fig E1
A, Absorbance curve of RNA isolated from EBC. B, A synthetic poly-A tail was added to miRNAs, followed by reverse transcription by using an oligo-dT linked to an adapter. Amplification of a panel of miRNAs by using a specific forward primer and a universal reverse primer to the adapter was analyzed by using qPCR. C, Raw data from qPCR amplification of miRNAs. D, Gel electrophoresis to confirm the expected size of miRNA PCR-products. E, The products of the gel were cloned and sequenced to confirm identity. A representative sequence is depicted for miR-21, showing the miRNA sequence, synthetic poly-A tail, and beginning of the 3′ adapter. qPCR, Quantitative PCR.
Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci )
Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4. Fig E2
A, Expression of small RNAs in EBC that can be used as internal standards (H, healthy; A, asthma; C, COPD). B, Standard curve generated by quantitative PCR of various dilutions of in vitro synthesized Cel-miR-39.
Journal of Allergy and Clinical Immunology  , e2DOI: ( /j.jaci )
Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions