1. Functional promoter activity of the human COX-2 gene in HMCs
Functional promoter activity of the human COX-2 gene in HMCs. A: A schematic diagram representing the wild-type pGL3-pCOX2 promoter and site-directed mutant constructs NF mut1, NF mut2, and NF mut1 and 2, used in transient transfection studies.
Functional promoter activity of the human COX-2 gene in HMCs. A: A schematic diagram representing the wild-type pGL3-pCOX2 promoter and site-directed mutant constructs NF mut1, NF mut2, and NF mut1 and 2, used in transient transfection studies. Mutated sites are represented by crosses. B: Cells were transiently transfected with pGL3-pCOX2 and were incubated with 5.6 mmol/l glucose, 30 mmol/l glucose alone, or 30 mmol/l glucose plus either UCP-1 or MnSOD. C: Cells were transiently transfected with pGL3-pCOX2 or mutated vectors NF mut1, NF mut2, or NF mut1 and 2, and were incubated with 5.6 (□) or 30 (▪) mmol/l glucose. Data are expressed relative to pGL3-pCOX2 of 5.6 mmol/l glucose and plotted as mean ± SE values. *P < 0.05 vs. pGL3-pCOX2-transfected cells incubated with 5.6 mmol/l glucose; #P < 0.05 vs. pGL3-pCOX2-transfected cells incubated with 30 mmol/l glucose; †P < 0.05 vs. NF mut1-transfected cells incubated with 30 mmol/l glucose (n = 6).
Shinsuke Kiritoshi et al. Diabetes 2003;52:
©2003 by American Diabetes Association