1. Fig. 3 Implantation of the battery-free optofluidic nerve cuff system and its impact on animal behavior and nerve health.
Implantation of the battery-free optofluidic nerve cuff system and its impact on animal behavior and nerve health. (A) Detailed illustration of the optofluidic nerve cuff system and cuff interface with the mouse sciatic nerve. (B) Demonstration of both optical stimulation and fluid delivery to the sciatic nerve. Scale bar, 2 mm. (C) Mouse chronically implanted with the wirelessly powered optofluidic nerve cuff system. (D) Characterization of effects of device implantation on rotarod performance compared to PE cuff and sham surgery [n = 9 to 10; ***P < PE cuff versus sham and device, two-way analysis of variance (ANOVA)]. Mouse gait parameters including maximum contact mean intensity (E), swing time (F), and print area (G) are significantly impaired after PE cuff implantation but not after optofluidic cuff implantation compared to sham surgery (n = 8 to 10; ***P < 0.001, **P < 0.01, and *P < 0.05, one-way ANOVA with Tukey’s multiple comparison). Representative hematoxylin and eosin images (H) and quantification of infiltrating immune cells (I) from the sciatic nerve comparing sham, device, and PE cuff after 2 weeks (w) of implantation demonstrating an absence of infiltrating immune cells in sciatic nerves of mice implanted with the device compared to PE cuff implantation. Scale bars, 25 μm. n = 3; ***P < 0.001, one-way ANOVA with Tukey’s multiple comparison. AU, arbitrary units.
Yi Zhang et al. Sci Adv 2019;5:eaaw5296
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).