1. MANF expression is required for maintaining β-cell mass.
MANF expression is required for maintaining β-cell mass. A–C: Quantitative RT-PCR for mRNA levels of β-cell–specific genes Glut2, Ins1/2, Pdx-1, MafA, and Gck in islets from P1, P14, and P56 pancreata (n = 6–11 per group). D and E: Quantitative RT-PCR analysis of UPR genes Atf4, Grp78, Chop, spXbp1, tXbp, and Atf6α and Atf6β in P1 and P14 islets from Pdx-1Cre+/−::Manffl/fl and Manffl/fl mice (n = 6–11 per group). F–N: Double IHC of MANF (I–K) and GLUT2 (F–H) in pancreatic sections from Manffl/fl (F, I, and L) and Pdx-1Cre+/−::Manffl/fl (G, H, J, K, M, and N) mice at P56. Cell nuclei were labeled with DAPI (blue). Scale bar = 10 μm. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < ns, not significant.
Tatiana Danilova et al. Diabetes 2019;68:66-80
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