1. An anti-BTC neutralizing antibody and a metalloproteinase inhibitor suppress GLP-1-induced DNA synthesis in INS(832/13) cells. [3H]thymidine incorporation measurements were carried out as described in Fig. 1.
An anti-BTC neutralizing antibody and a metalloproteinase inhibitor suppress GLP-1-induced DNA synthesis in INS(832/13) cells. [3H]thymidine incorporation measurements were carried out as described in Fig. 1. Cells were cultured at 3 mmol/l glucose for 24 h in RPMI medium in the absence or presence of 10 nmol/l GLP-1 with or without an anti-BTC antibody (BTC-Ab) (0.25 μg/ml) or 2.5 μmol/l GM6001 (llomastat), a pan-specific metalloproteinase inhibitor. Data represent means ± SE of three experiments, each comprising four wells. *P < 0.05 vs. control; **P < vs. GLP-1 treatment condition in the absence of inhibitor.
Jean Buteau et al. Diabetes 2003;52:
©2003 by American Diabetes Association