1. LATS2 augments oxidative phosphorylation.
LATS2 augments oxidative phosphorylation. (A) KEGG pathways enrichment analysis for genes differentially regulated in Lats2-CKO PyMT compared with WT-PyMT tumors (adjP-value < 0.05, n = 114). (B) ZR75-1 cells stably transfected with an MYC-LATS2 plasmid (LATS2) or with control vector were cultured with or without glucose for 3 d. Cell death was measured by PI exclusion followed by FACS analysis. Values from each experiment were normalized to % PI+ of control cells grown in glucose-containing medium; mean ± SEM of three independent experiments. (C) Extracellular acidification rate (ECAR, indicative of glycolysis) and oxygen consumption rate (OCR, indicative of respiration) of ZR75-1 cells stably expressing MYC-LATS2, relative to vector control cells, determined by Seahorse; mean ± SEM of five independent experiments. Representative tracks of Seahorse measurements are shown on the right. (D) Respiration measured by OCR in WT-PyMT and Lats2-CKO PyMT tumor-derived cell lines; mean ± SEM in log 10 scale of three independent experiments; P-value < Bottom: representative Seahorse track.
Noa Furth et al. LSA 2018;1:e
© 2018 Furth et al.