1. Volume 143, Issue 5, Pages 1144-1147 (November 2012)
Fixing the Breaks in Intestinal Stem Cells After Radiation: A Matter of DNA Damage and Death or DNA Repair and Regeneration 
Pauline Kay Lund 
Gastroenterology 
Volume 143, Issue 5, Pages (November 2012)
DOI: /j.gastro
Copyright © 2012 AGA Institute Terms and Conditions

2. Figure 1
(A) Location of 2 ISC populations, CBC and +4-ISC. Known or putative biomarkers of these 2 ISC populations. Lgr5, leucine-rich G-protein–coupled receptor 5; Olfm4, olfactomedin 4; Ascl2, acheate-scute complex homolog 2; Smoc2, SPARC-related modular calcium-binding protein 2; Sox9, Sry-box9; EGFP, enhanced green fluorescent protein; Bmi1, Bmi1 polycomb ring finger oncogene; mTert, mouse telomerase reverse transcriptase; Hopx, HOP homeobox; Lrig1, leucine-rich repeats and immunoglobulin-like domains 1; Dcamkl1, doublecortin-like kinase 1; LRC, label retaining cells, which show long-term retention of S-phase markers such as BrdU, are located predominantly at the +4 position and are putative ISC.4 Italicized biomarkers represent those validated by lineage tracing as marking multipotent ISC. In vivo multipotency of Sox9-expressing ISC has also been demonstrated by lineage tracing.20 However, as indicated, in a Sox9-EGFP reporter mouse, low-level reporter expression (Sox9-EGFPLow) marks CBC, whereas high-level expression (Sox9-EGFPHigh) marks crypt-based cells concentrated at the +4-ISC location.10 Dcamkl1 is a putative ISC biomarker which labels radiosensitive cells at the +4 position, that exhibit DNA damage after low-dose WBR.21 (B) Biomarkers of DNA damage and biomarkers of 2 different DNA repair pathways studied in Hua et al.7 (C) Time course of DNA damage, apoptosis, DNA repair, reentry into cell cycle, mitotic death, CBC expansion, and regeneration demonstrated for Lgr5+-CBC in Hua et al using an Lgr5-LacZ reporter mouse.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2012 AGA Institute Terms and Conditions