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A: Southern blot analysis of UCP-2 and GAPDH PCR products amplified from equal amounts of pancreatic islet total RNA isolated from control (n = 7) and HFD-fed animals (n = 8). A: Southern blot analysis of UCP-2 and GAPDH PCR products amplified from equal amounts of pancreatic islet total RNA isolated from control (n = 7) and HFD-fed animals (n = 8). B: The ratio of islet UCP-2 to internal control (GADPH) PCR products determined by computer-assisted imaging. Catherine B. Chan et al. Diabetes 2001;50: ©2001 by American Diabetes Association
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