1. Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to paclitaxel treatment.
Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to paclitaxel treatment. Representative Western blot (A) and densitometric quantitation (B) of the level of polymerized total tubulin (measured by α-tubulin levels) and polymerized modified βIII-tubulin (measured by GFP levels) in gene-edited NCI-H460 cells expressing either the full-length βIII-tubulin protein (ZB3), truncated βIII-tubulin (ZB3Δ), or βIII-tubulin body with the βI-tubulin C-terminal tail (ZB3/CB1) and treated with paclitaxel (6 or 20 nM for 4 h). Mean ± SEM of four independent experiments, no significant difference.
Amelia L Parker et al. LSA 2018;1:e
© 2018 Parker et al.