1. Inhibition of polymerization Enhancement of polymerization
Fig. S1 A B
IC50 = 26 nM C
Inhibition of polymerization
(1 mM GTP)
Enhancement of polymerization
(without GTP)
(mM)
Vehicle LY
Vincristine 0.1
Vincristine 1
Vincristine 3
-0.01
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0.08 10 20 30 40 50 60
Time (min)
Absorbance at 340 nm LY
Paclitaxel 3
Paclitaxel 10

2. Fig. S2 A B C
DNA
Pericentrin
Eg5
Merge
Control LY

3. Fig. S2 (cont.) D E Hours after Plating % Confluence 100nM 0nM 1.2nM-4 -3 -2 -1 1 10 20 30 40
3 h
1 h
6 h
12 h
18 h
24 h
Concentration (log mM)
Apoptotic Index (%) E

4. Caspase activity (% of control)
Fig. S3
DNA
pHH3 (S10)
pH2AX(S139)
Merge LY
Control A B
Mad2 + − 24 48 72
Mad2 siRNA
β-actin
Time (h) C D
Untreated
Control siRNA
Mad2 siRNA
LY Concentration
100
300
500
Caspase activity (% of control)
Untreated
Control siRNA
Mad2 siRNA

5. Fig. S4 A B a b c

6. C D

7. % of cancer cells positive for pHH3
Fig. S5
DNA
TUNEL
pHH3
Merge A
Control LY 5 10 15 20 25 30 35 40 1 2 3 4
Treatment Groups
% of cancer cells positive for pHH3 B