1. Effects of paclitaxel and vinblastine.
Effects of paclitaxel and vinblastine. (A) Average intensity of acetylated α-tubulin immunofluorescence in control cells (open bar) and patient cells (shaded bar) at baseline. Control cells expressed significantly more acetylated α-tubulin than patient cells. (B) Effects of paclitaxel (black lines) and vinblastine (grey lines) at 0-10 nM on acetylated α-tubulin immunofluorescence in control cells (continuous lines) and HSP patient cells (dashed lines). Both drugs significantly increased acetylated α-tubulin immunofluorescence, but paclitaxel more so (P<0.0001). Control cells were significantly larger than patient cells at nM paclitaxel (upper asterisks) and at 0-1 nM vinblastine (lower asterisks). (C) Average cell area calculated from acetylated α-tubulin immunofluorescence in control cells (open bar) and patient cells (shaded bar) at baseline. Control cells were significantly larger than patient cells. (D) Effects of paclitaxel (black lines) and vinblastine (grey lines) at 0-10 nM on cell area in control cells (continuous lines) and HSP patient cells (dashed lines). Both drugs reduced cell area in patient and control cells. Data indicate mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001.
Greger Abrahamsen et al. Dis. Model. Mech. 2013;6:
© Published by The Company of Biologists Ltd