1. MSHA is crucial for colonization of C. elegans by V. cholerae.
MSHA is crucial for colonization of C. elegans by V. cholerae. (A and B) Shown is the percentage of worms with detectable signal (dark gray) or no detectable signal (light gray) in the pharynx after grazing on V. cholerae WT pgfp or ΔmshA mutant pgfp before (A) or after (B) passage via E. coli OP50 for 2 h. At least 100 worms of each data set were analyzed. In both cases (A and B), the distributions of worms with and without signal are significantly different between nematodes grazing on V. cholerae WT pgfp and those grazing on ΔmshA mutant pgfp (*, P < 0.05 by Fisher exact test). (C) Shown are the colonization levels of worms grazing on V. cholerae WT or ΔmshA mutant after passage via E. coli OP50 for 2 h. Each circle represents the colonization level determined from 7 to 12 worms pooled for homogenization. The CFU were divided by the number of worms present in the respective homogenized pool to retrieve the CFU per worm (for details, see “Quantification of bacterial colonization” in Materials and Methods). The horizontal bar indicates the median of each data set. A significant difference is indicated by an asterisk (*, P < 0.05 by Mann-Whitney U test).
Cornelia List et al. mSphere 2018; doi: /mSphereDirect