1. Ub-binding ability of ERα.
Ub-binding ability of ERα. (A,C) Schematic of ERα (A, top) and ERα E (C, left) domain deletions are depicted. Amino acid positions and domains are indicated. In vitro pull-down assay using the indicated GST-tagged ERα constructs. GST-fusion proteins were incubated with total cellular lysates extracted from growing HeLa cells and analyzed by immunoblot as indicated. (B,D) As for A and C except that fusion proteins were incubated with synthetic polyUb2-7 linked by Lys63.
Valeria Pesiri et al. J Cell Sci 2013;126:
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