1. Affinities of the SV9 specific bs-868Z11-CD3 bsTCR with different MHC monomers and peptide ligands.
Affinities of the SV9 specific bs-868Z11-CD3 bsTCR with different MHC monomers and peptide ligands. (A) Binding curve of bs-868Z11-CD3 against immobilized DS-A*02:01 SL9 pMHC. Raw data are displayed in black and curve fittings are in red. Measured using 1:2 analyte dilution series starting at 500 nM. (B) Binding curve of bs-868Z11-CD3 against immobilized WT-A*02:01 SL9 pMHC. Raw data are displayed in black and curve fittings are in red. Measured using 1:2 analyte dilution series starting at 500 nM. (C) Correlation between affinities measured using DS-A*02:01 pMHCs or WT-A*02:01 pMHCs generated using UV exchange. Kd values were plotted for 140 different peptide ligands generated using both methods and measured during successive experiments. Kd values were fitted using 500 and 158 nM analyte concentrations. Peptides were included if peak signal levels reached at least 0.05 nM for both concentrations and individual fits had a R2 above 0.9. The in-picture R2 is the calculated correlation coefficient, and dashed line represents optimal 1:1 ratio.
Andreas Moritz et al. Sci. Immunol. 2019;4:eaav0860
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