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AKT thr308 phosphorylation related to total AKT2 protein in SOL muscle (m. soleus) (A) and EDL muscle (m. extensor digitorum longus) (D) and AKT ser472 phosphorylation related to total AKT2 protein in SOL muscle (B) and EDL muscle (E). AKT thr308 phosphorylation related to total AKT2 protein in SOL muscle (m. soleus) (A) and EDL muscle (m. extensor digitorum longus) (D) and AKT ser472 phosphorylation related to total AKT2 protein in SOL muscle (B) and EDL muscle (E). AS-160 phosphorylation in SOL muscle (C) and EDL muscle (F), in the presence of no insulin and during 100 µU/mL insulin stimulation at rest (white bars) and 90-min POST EX (PEX) (black bars) in WT and HSL KO mice. A representative blot is shown above each graph, measured between 16 and 25 weeks of age (n = 7–9 in each group). Data are expressed as the mean ± SEM. **Significant effect of 100 µU/mL insulin (P < 0.01). Annette Karen Serup et al. Diabetes 2016;65: ©2016 by American Diabetes Association
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