1. Temporal origins of h-ChCs labeled in Cdh6-CreER;Dlx5/6-Flp;Ai65 mice.
Temporal origins of h-ChCs labeled in Cdh6-CreER;Dlx5/6-Flp;Ai65 mice. A, Experimental timeline of BrdU-based birth dating. In Cdh6-CreER;Dlx5/6-Flp;Ai65 mice, h-ChCs were labeled by tamoxifen injection at P5, paired with a single BrdU administration at the indicated date. Mice were analyzed at P21. B, Confocal projection image of an RFP+ h-ChC (magenta) labeled with BrdU (green) administrated at E12. A confocal single optical section in inset shows the enlarged view of RFP and BrdU signals in a soma. C, Quantification of the percentage of BrdU+ cells among h-ChCs. h-ChCs are born between E11 and E14 with peaks at E12 and E13. (E10: 0%, n = 1 mouse, 27 ChCs; E11: 16.3 ± 3.5%, n = 3 mice, 60 ChCs; E12: 34.9 ± 2.7%, n = 4 mice, 151 ChCs; E13: 36.3 ± 4.7%, n = 3 mice, 68 ChCs; E14: 16.2 ± 3.7%, n = 3 mice, 61 ChCs; E15: 0%, n = 2 mice, 42 ChCs; E16: 0%, n = 3 mice, 81 ChCs). Data are presented as mean ± SEM. Scale bars: B, 50 μm; inset in B, 10 μm.
Yugo Ishino et al. J. Neurosci. 2017;37:
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