Presentation is loading. Please wait.

Presentation is loading. Please wait.

Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation  Masae Haga, MD, Akimasa Yamashita, MD, PhD, Jacek Paszkowiak,

Published byTyyne Pesonen Modified over 5 years ago

Similar presentations

Presentation on theme: "Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation  Masae Haga, MD, Akimasa Yamashita, MD, PhD, Jacek Paszkowiak,"— Presentation transcript:

1 Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation 
Masae Haga, MD, Akimasa Yamashita, MD, PhD, Jacek Paszkowiak, MD, Bauer E Sumpio, MD, PhD, Alan Dardik, MD, PhD  Journal of Vascular Surgery  Volume 37, Issue 6, Pages (June 2003) DOI: /S (03)00329-X

2 Fig 1 Increased smooth muscle cell (SMC) proliferation in response to oscillatory shear stress (SS). Synchronized SMC were exposed to oscillatory SS (14 dyne/cm2) for up to 5 days or to static conditions, and cell numbers were counted with an automatic counter. A, Representative experiment. Primary group, number of SMC immediately after seeding and before synchronization with serum withdrawal for 24 hours; 0 day, primary group after synchronization and at start of SS exposure. B, Summary of three independent experiments. Error bars reflect SEM. Journal of Vascular Surgery  , DOI: ( /S (03)00329-X)

3 Fig 2 Increased smooth muscle cell DNA synthesis in response to oscillatory shear stress. Synchronized smooth muscle cells were exposed to oscillatory shear stress (14 dyne/cm2) for up to 5 days or to static conditions in the presence of 3[H]thymidine; trichloroacetic acid–precipitable protein was counted with a scintillation counter. A, Representative experiment. B, Summary of three independent experiments. Error bars reflect SEM. Journal of Vascular Surgery  , DOI: ( /S (03)00329-X)

4 Fig 3 Time course of Akt phosphorylation by shear stress in smooth muscle cells. Top, Photomicrograph of representative experiment. Bottom, Bar graph depicts mean increase in ratio of phosphorylated to total Akt, compared with baseline (time 0). Error bars reflect SEM. Difference between groups is significant (n = 4; P = .01, Kruskal-Wallis test). Journal of Vascular Surgery  , DOI: ( /S (03)00329-X)

5 Fig 4 Specificity of Akt phosphorylation in smooth muscle cells. Top, Photomicrograph of representative experiment. Bottom, Bar graph depicts mean increase in ratio of phosphorylated to total Akt, compared with static conditions (shear stress, 0 dyne/cm2). Error bars reflect the SEM. Difference between groups exposed to shear stress, with or without LY or wortmannin, is significant (n = 2; P < .05, Mann-Whitney U test). Journal of Vascular Surgery  , DOI: ( /S (03)00329-X)

Download ppt "Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation  Masae Haga, MD, Akimasa Yamashita, MD, PhD, Jacek Paszkowiak,"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages 1072-1082 (October 2014) DOI:

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages (October 2014) DOI:
R. Abe, J. Beckett, R. Abe, A. Nixon, A. Rochier, N. Yamashita, B

R. Abe, J. Beckett, R. Abe, A. Nixon, A. Rochier, N. Yamashita, B
Graft smooth muscle cells specifically synthesize increased collagen

Graft smooth muscle cells specifically synthesize increased collagen
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis 

Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis 
Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.

Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.
Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 

Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 
Patrick C. H. Hsieh, MD, Richard D. Kenagy, PhD, Eileen R

Patrick C. H. Hsieh, MD, Richard D. Kenagy, PhD, Eileen R
Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.
Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,

Differential effects of orbital and laminar shear stress on endothelial cells  Alan Dardik, MD, PhD, Leiling Chen, MD, Jared Frattini, MD, Hidenori Asada,
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.

Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.
Effect of tissue plasminogen activator on vascular smooth muscle cells

Effect of tissue plasminogen activator on vascular smooth muscle cells
Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,
Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,

Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,
Bertrand Poussier, MD, Alfredo C

Bertrand Poussier, MD, Alfredo C
Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.

Role of redox signaling and poly (adenosine diphosphate-ribose) polymerase activation in vascular smooth muscle cell growth inhibition by nitric oxide.
Domain-dependent action of urokinase on smooth muscle cell responses

Domain-dependent action of urokinase on smooth muscle cell responses
Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,
Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.
The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling 

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling 

Similar presentations

Ads by Google