1. Low-Energy Helium-Neon Laser Induces Locomotion of the Immature Melanoblasts and Promotes Melanogenesis of the More Differentiated Melanoblasts: Recapitulation of Vitiligo Repigmentation In Vitro 
Cheng-Che E. Lan, Ching-Shuang Wu, Min-Hsi Chiou, Pei-Chen Hsieh, Hsin-Su Yu 
Journal of Investigative Dermatology 
Volume 126, Issue 9, Pages (September 2006)
DOI: /sj.jid
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Effect of He-Ne laser irradiation on NCCmelb4 and NCCmelan5 cell growth. The growth curve of (a) NCCmelb4 and (b) NCCmelan5 constructed from results of cell counting and trypan blue dye exclusion assay showed that He-Ne laser irradiation up to 1.5J/cm2 did not significantly alter the cell numbers of either cell types up to 5 days of cultivation. The data shown here (mean±SD) are from one representative experiment performed in triplicate, repeated three times with similar results.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Effect of He-Ne laser irradiation on cell attachment to fibronectin. The NCCmelb4 and NCCmelan5 cells (1 × 104/well) were seeded in 96-well microtitration plate coated with fibronectin. Attached cells were determined following the experimental protocol described in the Materials and Methods section. As seen in the figure, 1.0J/cm2 He-Ne laser irradiation significantly decreased the number of NCCmelb4 cells attached to fibronectin. On the other hand, He-Ne laser irradiation significantly increased the number of NCCmelan5 cells attached to fibronectin. The data shown here (mean±SD) are from one representative experiment performed in triplicate, repeated three times with similar results. *P<0.05 as compared to the 0J/cm2 irradiated group.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Effect of He-Ne laser irradiation on pp125FAK expression of the NCCmelb4 cells. The relative expression of pp125FAK on the NCCmelb4 cells with or without 1.0J/cm2 He-Ne laser irradiation was obtained by densitometric scanning of the representative immunoblot shown in the figure after adjusting for internal control, glyceraldehyde-3-phosphate dehydrogenase. The relative intensity of pp125FAK significantly increased after 1.0J/cm2 He-Ne laser irradiation. The data shown is a representative blot from three separate experiments with similar results, and the densitometric analyses (mean±SD) are obtained from three independent experiments. *P<0.05 as compared to the 0J/cm2 irradiated group.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Effects of specific inhibitors on locomotion of the NCCmelb4 cells. The NCCmelb4 cells were seeded onto fibronectin-coated Cellocate slips at 2 × 104 cells/dish. Time-lapse photographs were taken at indicated time points and the migration distance (μm) was determined. As shown previously, treatment with 1.0J/cm2 He-Ne laser significantly enhanced the locomotion of the NCCmelb4 cells. HerbimycinA, a specific pp125FAK antagonist abrogated the enhancing effect of He-Ne laser on NCCmelb4 cells. On the other hand, neutralizing antibody against α5β1 integrin has no significant impact on He-Ne laser-induced NCCmelb4 locomotion. The data shown here (mean±SD) are from one representative experiment, repeated three times with similar results. *P<0.05 as compared to the 1.0J/cm2 He-Ne laser-irradiated group.
Journal of Investigative Dermatology  , DOI: ( /sj.jid )
Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions