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Production of macrophage inflammatory protein-3α in human follicular fluid and cultured granulosa cells  Yasushi Kawano, M.D., Junichiro Fukuda, M.D.,

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Presentation on theme: "Production of macrophage inflammatory protein-3α in human follicular fluid and cultured granulosa cells  Yasushi Kawano, M.D., Junichiro Fukuda, M.D.,"— Presentation transcript:

1 Production of macrophage inflammatory protein-3α in human follicular fluid and cultured granulosa cells  Yasushi Kawano, M.D., Junichiro Fukuda, M.D., Kaei Nasu, M.D., Masakazu Nishida, M.D., Hisashi Narahara, M.D., Isao Miyakawa, M.D.  Fertility and Sterility  Volume 82, Pages (October 2004) DOI: /j.fertnstert Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 The concentrations of MIP-3α in follicular fluid aspirated from various follicles during in vitro fertilization cycles. Oocytes are classified as immature, intermediate, or mature. Follicular fluid from which no oocytes were recovered is indicated as “undetected.” *P <.05 vs. immature (Bonferroni/Dunn test). Data are expressed as mean ± SE. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 The levels of MIP-3α in the culture media of GC1a after 24 hours stimulation with IL-1α (1 nM), IL-1ra (10 nM), and TNF-α (1 nM). *P <0.01 vs. unstimulated control, ** P <.05 vs. IL-1α (1 nM) stimulation (Bonferroni/Dunn test). The data are expressed as mean ± SD of triplicate samples from a representative experiment. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 The levels of MIP-3α in the culture media of GC1a after 4 to 32 hours of stimulation with IL-1α (1 nM) and TNF-α (1 nM) (A). Open square; unstimulated control, closed circle; IL-1α (1 nM) stimulation, closed triangle; TNF-α (1 nM) stimulation. The levels of MIP-3α in the culture media of GC1a after 24 hours of stimulation with IL-1α at concentrations of 1 pM to 10 nM (B), IL-1α (1 nM) and IL-1ra at concentrations of 10 pM to 10 nM (C), and TNF-α (D) at concentrations of 1 pM to 10 nM, respectively. * P<.01, ** P<.001 vs. unstimulated control, *** P<.01 vs. IL-1α (1 nM) stimulation. The data are expressed as mean ± SD of triplicate samples from a representative experiment. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions


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