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Functional characterization of CD4+ T-cells with various antigen specificities after transduction with FoxP3. Functional characterization of CD4+ T-cells.

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Presentation on theme: "Functional characterization of CD4+ T-cells with various antigen specificities after transduction with FoxP3. Functional characterization of CD4+ T-cells."— Presentation transcript:

1 Functional characterization of CD4+ T-cells with various antigen specificities after transduction with FoxP3. Functional characterization of CD4+ T-cells with various antigen specificities after transduction with FoxP3. A: Expression of FoxP3 mRNA by RT-PCR. β-Actin cDNA was amplified as an internal control. B: In vitro proliferation assay in response to anti-CD3 stimulation of polyclonal-naïve CD4+CD25− cells (white bar) and polyclonal (CD4+) (light gray bar) p286 of GAD65 (GAD)-specific (dark gray bar) and CD4+ cells from BDC2.5 mice (black bar) after retroviral transduction with eGFP control virus or FoxP3. C: Coculture of 2 × 104 CD4+CD25− cells with various numbers of FoxP3-transduced polyclonal (CD4), GAD p286–specific (GAD), and BDC2.5 cells. D: IL-10 production in response to anti-CD3 stimulation of polyclonal (CD4), GAD p286–specific (GAD), and BDC2.5 cells without retroviral transduction and after transduction with eGFP control virus and FoxP3. Elmar Jaeckel et al. Diabetes 2005;54: ©2005 by American Diabetes Association

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