1. c-Fos in RFMes GABAergic neurons across groups.
c-Fos in RFMes GABAergic neurons across groups. Fluorescent microscopic images show staining for Nissl with FNS (green; A1, B1, C1), immunostaining for GAD (blue; A2, B2, C2, with positive staining indicated by filled arrowheads), and immunostaining for c-Fos (red; A3, B3, C3, with positive staining indicated by filled arrowhead) along with dual staining for Nissl and c-Fos in merged images (green and red; A4, B4, C4, with positive c-Fos staining indicated by filled arrowhead). Note that c-Fos immunostaining is prominent in the nucleus of a GABAergic neuron from an SD mouse (B3, B4), whereas it is not apparent in images from SC or SR mice (A3, A4 or C3, C4, indicated by open arrowheads). Scale bars: 20 μm. Image thickness: 500 nm in all panels.
Hanieh Toossi et al. eNeuro 2017;4:ENEURO
©2017 by Society for Neuroscience