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Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy  Esther C. de Jong, MSca, b, Steven Spanhaak,

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Presentation on theme: "Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy  Esther C. de Jong, MSca, b, Steven Spanhaak,"— Presentation transcript:

1 Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy  Esther C. de Jong, MSca, b, Steven Spanhaak, MSca, Ben P.M. Martens, MDc, Martien L. Kapsenberg, PhDd, André H. Penninks, PhDa, Eddy A. Wierenga, PhDd  Journal of Allergy and Clinical Immunology  Volume 98, Issue 1, Pages (July 1996) DOI: /S (96) Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 Proliferative response of PBMCs from patients with peanut allergy (PA, n = 7) and nonallergic donors (NA, n = 10) to CPE (A) and to PNA (B). Proliferation of PBMCs (2 × 105 cells/well) was determined by measuring [3H]-TdR incorporation after 7 days of incubation in the absence or presence of increasing amounts of CPE or PNA. Results are expressed as mean stimulation index (SI) ± SEM. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 1 Proliferative response of PBMCs from patients with peanut allergy (PA, n = 7) and nonallergic donors (NA, n = 10) to CPE (A) and to PNA (B). Proliferation of PBMCs (2 × 105 cells/well) was determined by measuring [3H]-TdR incorporation after 7 days of incubation in the absence or presence of increasing amounts of CPE or PNA. Results are expressed as mean stimulation index (SI) ± SEM. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 2 CPE-specific proliferative responses of T-cell clones from peanut-allergic patient NvD. Cloned cells (105 cells/well) were cultured with rad irradiated autologous PBMCs as APCs in the absence or presence of 50 μg/ml CPE. Proliferation was measured after 48 hours of incubation with [3H]-TdR incorporation. Bars express mean values ± SEM of triplicate cultures, and stimulation index is given on top of the bars. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

5 FIG. 3 Cytokine secretion by CPE-specific T-cell clones. Cloned cells were stimulated (105 cells/well) for 36 hours with anti-CD3 plus anti-CD28 (both, 1:1000 final dilution), after which the cytokine concentrations in the culture supernatants were measured by ELISA. Secretion levels were compared with those of well-characterized aeroallergen-specific T h1 (MBA.AA40 and MBE.AA42) and T h2 (MBB.AA60) clones tested identically. Detection levels: IFN-γ, 20 pg/ml; IL-4, 2.4 pg/ml; IL-5, 0.2 ng/ml. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

6 FIG. 3 Cytokine secretion by CPE-specific T-cell clones. Cloned cells were stimulated (105 cells/well) for 36 hours with anti-CD3 plus anti-CD28 (both, 1:1000 final dilution), after which the cytokine concentrations in the culture supernatants were measured by ELISA. Secretion levels were compared with those of well-characterized aeroallergen-specific T h1 (MBA.AA40 and MBE.AA42) and T h2 (MBB.AA60) clones tested identically. Detection levels: IFN-γ, 20 pg/ml; IL-4, 2.4 pg/ml; IL-5, 0.2 ng/ml. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

7 FIG. 3 Cytokine secretion by CPE-specific T-cell clones. Cloned cells were stimulated (105 cells/well) for 36 hours with anti-CD3 plus anti-CD28 (both, 1:1000 final dilution), after which the cytokine concentrations in the culture supernatants were measured by ELISA. Secretion levels were compared with those of well-characterized aeroallergen-specific T h1 (MBA.AA40 and MBE.AA42) and T h2 (MBB.AA60) clones tested identically. Detection levels: IFN-γ, 20 pg/ml; IL-4, 2.4 pg/ml; IL-5, 0.2 ng/ml. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

8 FIG. 3 Cytokine secretion by CPE-specific T-cell clones. Cloned cells were stimulated (105 cells/well) for 36 hours with anti-CD3 plus anti-CD28 (both, 1:1000 final dilution), after which the cytokine concentrations in the culture supernatants were measured by ELISA. Secretion levels were compared with those of well-characterized aeroallergen-specific T h1 (MBA.AA40 and MBE.AA42) and T h2 (MBB.AA60) clones tested identically. Detection levels: IFN-γ, 20 pg/ml; IL-4, 2.4 pg/ml; IL-5, 0.2 ng/ml. Journal of Allergy and Clinical Immunology  , 73-81DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

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