1. PROTEIN-PROTEIN INTERACTIONS
July 15th, 2009
D.M. Syracuse, Presenter

2. Troubleshooting a screen
Need to select a protein from a whole library of proteins
Use an antibody-antigen pair to select them.
However, unexpected binding is occurring

3. What I’m working on
I have some proteins (parts of antibodies) that I know will bind to the antigen.
I have some that I know will not bind.
The proteins are fused to GFP – their presence can be detected.
I’ve made and expressed plasmids in E. coli with the various proteins in them.

4. What it can lead to
A high throughput filter to screen a library for proteins.
Potential for quicker research, no need for sequencing, etc.