1. Macaque Satb2-RGCs are synaptically connected to geniculate neurons.
Macaque Satb2-RGCs are synaptically connected to geniculate neurons. A, Schematic demonstrating injection of G-deleted rabies virus (Rb-ΔG) encoding fluorescent proteins in the dLGN. A′, A″, Local infection/spread of Rb-ΔG-GFP (green) within the dLGN (A″). dLGN layers visualized by VGlut2 staining (A′, white). B–B″, Injection of Rb-ΔG-GFP in dLGN results in infection of RGCs (B) that form synapses with geniculate neurons. Satb2 (magenta, B′) is expressed by some dLGN projecting RGCs (green, B). White arrowheads indicate colocalization of GFP and Satb2 and dashed circles indicate lack thereof. C, Example en face view of Satb2-RGC morphology recovered from rabies GFP infection. D–D″, Higher magnification of Satb2-RGC soma (D, green) expressing Satb2 (D′, magenta). E, 3D reconstruction of Satb2-RGC dendritic morphology (black) shown in C. Soma and axon shown in red. XFP: GFP or mCherry fluorescent protein; M, magnocellular layer; P, parvocellular layer. Scale bars, 1 mm (A″), 25 μm (B″), 100 μm (C, E).
Onkar S. Dhande et al. J. Neurosci. 2019;39:78-95
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