1. Synapse composition is unaltered in SynDIG1-deficient synapses.
Synapse composition is unaltered in SynDIG1-deficient synapses. A, Representative immunoblots of biochemical fractions isolated from WT and SynDIG1β-gal homozygous mutant P14 mouse brain tissue showing levels of GluA1, GluA2, GluN1, GluN2B, PSD-93, PSD-95, synaptophysin (Synapto), SynDIG1, SynDIG4, and PICK-1 present in the S1-, P2-, Syn-, and PSD-enriched fractions. Loading controls are provided by β-actin and β-tubulin immunoreactivity. B–D, Graphs depict the ratio of SynDIG1β-gal homozygous mutant protein relative to WT levels of AMPA receptor subunits (B), NMDA receptor subunits (C), and PSD-93 and PSD-95 (D) in the PSD-enriched fractions. Data are the average of three independent biochemical fractionation experiments; each experiment used four to six mouse brains of each genotype. Error bars represent ±SEM.
George Chenaux et al. eneuro 2016;3:ENEURO
©2016 by Society for Neuroscience