1. Neuronal and glial expression of Nogo-A and synaptic localization of Nogo-A and NgR 1 in the motor cortex.
Neuronal and glial expression of Nogo-A and synaptic localization of Nogo-A and NgR 1 in the motor cortex. A, A′, Overview of a Nogo-A-stained brain section (A) and a control section in absence of the primary Ab (A′). Scale bar, 1 mm. A1, A2, Magnified images of the motor cortex stained for Nissl (A1) and Nogo-A (A2). Cortical layers are indicated in A1. Scale bar, 200 μm. B–G, Immunostaining against Nogo-A revealed expression of the protein in layer 2/3 cells (B, C), layer 5 neurons (D, E), and glial cells of the corpus callosum (F, G). Note the brighter immunofluorescence and higher Nogo-A content in the oligodendrocytes compared with the neurons. C, E, G, Overlay of Nogo-A/Nissl staining. Scale bar, 15 μm. H–M, Single-plane confocal images of the neuropil at high magnification immunostained for NgR1 (H), synaptophysin (I), and overlay (J), as well as for Nogo-A (K), synaptophysin (L), and overlay (M). Insets in J and M show merged images at a higher resolution. Scale bar, 5 μm. N–P, Representative electron microscopic images showing Nogo-A (N), the presynaptic marker vGlut-1 (O), and NgR1 (P) immunoreactive gold particles in and on the postsynaptic, extrasynaptic, and presynaptic membranes and compartments. Quantification of these results is shown in Table 1. Scale bars, 100 nm. S, Postsynaptic spine, T, presynaptic terminal.
Ajmal Zemmar et al. J. Neurosci. 2014;34:
©2014 by Society for Neuroscience