1. Interaction of full-length TcdB with cells.
Interaction of full-length TcdB with cells. (A) Cartoon illustrating the TcdB and TcdB fragments used in this study. GTD, glucosyltransferase domain; CPD, cysteine protease domain; CROP, combined repetitive oligopeptides. (B) Summary of flow cytometry of CHO-K1 cells stained with Alexa Fluor 488-labeled full-length TcdB1 (open circles) or TcdB2 (closed squares). P < 0.01 for all concentrations of TcdB2 versus TcdB1. MFI, mean fluorescence intensity. (C) Western blotting of whole-cell lysates representative of six independent experiments evaluating the binding of 0.37 nM full-length TcdB1 and TcdB2 to CHO-K1 and Vero cells. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (D) Quantification by densitometry of the blots in panel C.
Jonathan J. Hunt et al. mSphere 2017; doi: /mSphere