1. CD25 expression identifies two transcriptionally distinct subsets of very early effector cells.
CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. (A) CD25hi and CD25lo SMARTA cells were isolated at day 5 post-infection, followed by RNA-seq (n = 3). Hierarchical clustering indicated unique gene expression patterns and significantly up-regulated (red) or down-regulated (blue) genes. (B) Bar graph indicates a list of selected genes with significantly increased expression in CD25hi (red) and CD25lo (blue) populations. The x axis indicates the difference in the number of transcripts on a log2 scale. (C) Bar graphs show RT-PCR–based confirmation of differences in gene expression for the selected genes between day 5 CD25hi (“High”) and CD25lo (“Low”) SMARTA CD4+ T cells. SEM (n = 3 samples per group). *P < 0.05, **P < 0.01, ***P <
Jeremy P. Snook et al. Sci. Immunol. 2018;3:eaas9103
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