1. Uptake of amino acids by C. albicans.
Uptake of amino acids by C. albicans. The wild type and strains lacking one of the GAP gene family members or lacking 3 GAP genes (GAP1, GAP2, and GAP6) were used. Transport of 2 mM citrulline (black bars), leucine (dark gray bars), phenylalanine (light gray bars), or glutamine (white bars) was measured using cells which were incubated under nitrogen starvation conditions (without any source of nitrogen) (A) or growing under nitrogen-rich conditions (with ammonium sulfate as nitrogen source) (B). The level of transport is expressed as nanomoles of amino acid transported per minute per milligram of protein, and statistical significance (*) was determined by comparison with the wild type.
Lucie Kraidlova et al. mSphere 2016; doi: /mSphere