1. Class 1 phosphatidylinositol 3-kinase (PI3K) p85 subunit dominant negative adenovirus regulates Pax2 and GAPDH abundance in NRK-52E cells.
Class 1 phosphatidylinositol 3-kinase (PI3K) p85 subunit dominant negative adenovirus regulates Pax2 and GAPDH abundance in NRK-52E cells. NRK-52E cells were infected with either green fluorescence protein (GFP) or p85 dominant negative adenoviruses (p85 DN) at a series of dosages for 48 h. The multiplicities of infection (MOI) of p85 DN were approximately 10 plaque-forming units (pfu)/cell, approximately 30 pfu/cell, and approximately 90 pfu/cell. Western analysis was carried out by using the same amount of protein extracts (10 μg). The statistical analysis results are shown. (A) Cells were treated with EGF (E; 10 nM) or vehicle (C) for 15 min. Immunoblot analysis was performed by using antibody against phospho-Akt (Ser473; n = 3). (B) Cells were treated as indicated for 96 h. Immunoblot analysis was performed by using antibody against Pax2 (n = 4). (C) Cells were treated as indicated for 48 h. Immunoblot analysis was performed by using antibody against GAPDH (n = 4). (D) Immunoblotting with antibody against actin (n = 4).
Wen Shen et al. JASN 2006;17:
©2006 by American Society of Nephrology